Penelitian

Carotenoids from Female Sally Lightfoot Crab Carapace as Potential Anti-Sars-CoV-2 Compounds

DARUS SAADAH J. PARANSA;   DESY MARIA HELENA MANTIRI;   KURNIATI KEMER;  
Publish : 2020 | -


During the Covid-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the search for potential compounds from natural materials is needed. Carotenoids are one of very potent natural antioxidants, one of which can be found in marine animals. Several studies have shown that antioxidants such as carotenoids have antiviral activity. Crabs that live on the rocky shore such as Sally lightfoot are a group of crustaceans that have bright and attractive colour on their carapace which indicates most likely due to the presence of carotenoids. This study aimed to characterize the types of carotenoid pigments found in the carapace of female Sally lightfoot crab (Grapsus albolineatus) and evaluate their potentials as Sars-CoV-2 inhibitors. The crab used in this study was in the D3 molting stage. Analysis of the type of carotenoid pigments was carried out using column chromatography separation with petroleum ether (PE) and acetone (PE/A) as developing solution in a ratio of 80:20 and 70:30. Isolation of the pigment extract was continued by separation method using High Performance Liquid Chromatography (HPLC). Separation of column chromatography using PE/A with a ratio of 80:20 produced the following pigments: β-carotene, canthaxanthin and adonirubin. The pigments β-carotene, echinenone, canthaxanthin, astaxanthin, and astacene were found in the total pigment extract of female G. albolineatus Crab carapace from the results of the separation of column chromataography with a ratio of PE/A 70:30. The results of further analysis on the extracts of astaxanthin pigments through HPLC showed the presence of astaxanthin and tetrahydroastaxanthin pigments. The results of molecular docking analysis revealed that carotenoids from the carapace of female G. albolineatus have high binding free energies against spike glycoprotein (closed state), spike ectodomain structure (open state) and receptor binding domain of Sars-CoV-2.

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